In immuno - blotting , these fragments reacted specifically with hepatitis c patients " sera , suggesting that e . coli - derived e2 proteins carried hcv e2 - specific , glycosylation - and - conformation - independent epitopes 在免疫印迹检测中,上述片段与丙型肝炎病人血清有特异性反应,表明大肠杆菌系统表达的e2蛋白携带有hcve2特异的、不依赖于糖化和立体构象的抗原决定簇。
By immunizing rabbits with aa 192 - 326 fragment , polyclonal sera were obtained that were able to recognize e1 proteins expressed in both e . coli and mammalian cells , suggesting that e . coli - derived e2 proteins carried hcv e1 - specific , glycosylation - and - conformation - independent epitopes 利用aa192 - 326免疫家兔,获得了可识别大肠杆菌和哺乳动物细胞表达之e1蛋白的多抗血清,表明大肠杆菌系统表达的e1蛋白携带有hcve1特异的、不依赖于糖化和立体构象的抗原决定簇。