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fixative solution中文是什么意思

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  • Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces
    2 )磷酸缓冲液100400m , 30分钟灌注完毕,取出整脑,在上述固定剂oc )内后固定12小时。切取观察部位脑块,然后,进行梯度酒精脱水,浸蜡,包埋,修块,石蜡连续切片(德国leica石蜡切片机人切片厚度still , zlllll ,蛋自甘油载片捞片, 60c烤箱过夜,二甲苯脱蜡,梯度酒精置换,浸水, h六染色,梯度酒精脱水,二甲苯透明,中性树脂封片。室温风干后,显微镜观片, olympus万能显微镜照相。
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Last modified time:Tue, 19 Aug 2025 00:29:56 GMT

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