Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish . the construction and evalution of the a , gtlo cdna library of grass carp leukocytes are described . total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction 本文第一部分取病毒感染36小时后的草鱼头肾并分离白细胞,用改进的异硫氰酸胍一步法从其中提取总rna ,磁珠法分离纯化mrna ,电泳检测其质量。