In this experiment, the lung cells of embryo sheep were cultured and the cells were infected with jsrv 将绵羊的胚胎肺细胞进行原代培养,接种绵羊肺腺瘤样品,进行病毒分离。
Studies on jsrv had been hampered by the lack of an infectious molecular jsrv clone and the lack of an in vitro infection system 据报道,此病毒不易在体外培养,本试验对建立本病毒的体外培养体系进行了探索。
Studies on jsrv had been hampered by the lack of an infectious molecular jsrv clone and the lack of an in vitro infection system 据报道,此病毒不易在体外培养,本试验对建立本病毒的体外培养体系进行了探索。
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants . exjsrv-specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus-specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences 用地高辛随机引物法标记exjsrv特异的env片段,制备探针,原位杂交检测spa肺组织中的rna及前病毒dna,结果表明spa患羊肺组织内有jsrvenv基因mrna的表达,同时也检测到了前病毒dna,而相应的阴性对照却无阳性信号,证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants . exjsrv-specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus-specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences 用地高辛随机引物法标记exjsrv特异的env片段,制备探针,原位杂交检测spa肺组织中的rna及前病毒dna,结果表明spa患羊肺组织内有jsrvenv基因mrna的表达,同时也检测到了前病毒dna,而相应的阴性对照却无阳性信号,证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。
Sheep pulmonary adenomatosis ( spa ) is a contagious and experimentally transmissible lung cancer of sheep resembling human bronchiolo-alveolar carcinoma, spa is a nature animal model in study of bac . a type d retrovirus, known as jaagiekte sheep retrovirus ( jsrv ), has been associated with the etiology of, spa 绵羊肺腺瘤病(sheeppulmonaryadenomatosisspa)是一种传染性肺癌,并可实验复制。spa与人的支气管上皮细胞癌(bronchioloalveolarcarcinomabac)极为相似,是研究bac的天然动物模型。
For the first time, the complete long env gene of the jaagsiekte sheep retrovirus ( jsrv ) has been colpned from provimses in china in sheep genome and sequeneed, the gene is 1836 nucleotides long . there are 12 nucleotides mutations compared with type i and type ii jsrv counterparts the sequences of the virus was 87 % to 88 % identical to the described three jsrv env sequences 将pcr产物纯化,并与puem-t载体相连,转化大肠杆菌,经pcr,酶切鉴定及序列测定,得知所得env片段与型jsrv同源性为88,与型jsrv同源性为87,证明所克隆基因为外源性目的基因,至此,我们在国内首次获得了env阳性克隆。
For the first time, the complete long env gene of the jaagsiekte sheep retrovirus ( jsrv ) has been colpned from provimses in china in sheep genome and sequeneed, the gene is 1836 nucleotides long . there are 12 nucleotides mutations compared with type i and type ii jsrv counterparts the sequences of the virus was 87 % to 88 % identical to the described three jsrv env sequences 将pcr产物纯化,并与puem-t载体相连,转化大肠杆菌,经pcr,酶切鉴定及序列测定,得知所得env片段与型jsrv同源性为88,与型jsrv同源性为87,证明所克隆基因为外源性目的基因,至此,我们在国内首次获得了env阳性克隆。
For the first time, the complete long env gene of the jaagsiekte sheep retrovirus ( jsrv ) has been colpned from provimses in china in sheep genome and sequeneed, the gene is 1836 nucleotides long . there are 12 nucleotides mutations compared with type i and type ii jsrv counterparts the sequences of the virus was 87 % to 88 % identical to the described three jsrv env sequences 将pcr产物纯化,并与puem-t载体相连,转化大肠杆菌,经pcr,酶切鉴定及序列测定,得知所得env片段与型jsrv同源性为88,与型jsrv同源性为87,证明所克隆基因为外源性目的基因,至此,我们在国内首次获得了env阳性克隆。