coding sequence造句
例句与造句
- The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
将克隆到的hbmp基因通过适当的酶切插入到转移载体质粒pbac - pak8的多克隆位点中，获得重组转移载体质粒pbacpak - hbmp 。 - Based on the single cycle detector , the finite cycle detector is proposed for the detection of phase - coded signals . the finite cycle detector has a good performance under unknown the spreading code sequence , initial phase , spreading code timing epoch
针对相位编码信号的检测，提出了基于单循环检测器的有限多循环检测器，可在没有编码序列、初相、编码序列初始时间等信息的情况下，取得理想的检测效果。 - On the basis of the composition of a file , the sequence of significant code sequences or based on particular behavior patterns , the heuristics can determine with a high probability whether it is dealing with a harmful or virulent file
在文件的基层结构（不知道怎么翻译- _ - | | ） ，有意义代码的顺序或者基于特殊的行为特征（还是不知道怎么翻译- _ - | | ） ，有很高的几率可以启发出文件是否是一个有害的或者含有病毒的文件。 - Every class endues a binary code , then a set of svms are used to solve the multiple binary problems . the generalization performance of ecc - svm is analyzed , which is determined by code length , hamming distance , coding sequence and margins of svms
本文提出了基于纠错编码的svm多类分类算法( ecc - svm ) ，并分析了ecc - svm的推广能力与编码长度、码间汉明距离、编码顺序以及分类间隙等之间的关系，给出了这种关系的数学描述。 - Fasl - ecd coding sequence is subcloned into pet - lla expressing vector , recombinant expression vector are named as pet - fasl - ecd . this plasmid is introduced into e . coli bl21 . after induction with 1mmol / l iptg , the protein expression is analyzed and confirmed by coomassie - stained sds - page
用pcr和酶切鉴定的方法筛选出阳性重组子，将阳性重组子以ilnlnol几iptg进行诱导表达，以sds一page分析fasl胞外区的表达。 - It's difficult to find coding sequence in a sentence. 用coding sequence造句挺难的
- A 12 bp sequence of the 5 " end from the polyhedrin protein gene of bmnpv was ligated to the 5 ' end of hbsag ( pres2 + s ) protein coding sequence by pcr . the fusion product coding for hbv surface antigen medium sized ( hbmp ) with 4 - additional aa of bmnpv polyhedrin protein was obtained
本研究通过pcr突变的方法，在hbsag ( pres2 + s )前s2序列的5 ’端融合了bmnpv多角体蛋白基因5 ’端的12个碱基，获得了融合乙肝表面抗原中蛋白基因( hbmp ) 。 - The nj map and simplest principle map based on 3 ' end coding sequences are identical to the two maps based on the complete coding sequences . this shows that 3 " end coding sequences of epsp can replace the complete coding sequences to analyze the molecular evolution
用epsps基因全长编码区的dna序列构建的nj图，最简约树与用其3 ’端编码区dna序列构建的树形图相同，表明完全可以用epsps基因3 ’端编码区dna序列代替基因全长编码序列作进化分析。 - Hla - g1 , which is a newly defined non - classical hla class i molecule , plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking . the full - length coding sequences containing cdna of hla - g1 were cloned from placenta , monocytes and liver cancer tissue of chinese donors . sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality . its truncated form was overexpressed in
从中国人外周血单个核细胞胎盘组织和肝癌组织等样品中克隆了包含完整hla - g1读框的cdna与国外同行获得的该基因及其蛋白质序列比较分析表明，该基因虽然有着细微的种族特异性，但高度保守并获得了它的截断型重组蛋白，根据蛋白一级结构和同源比较方法，模建了它及其与特异性受体kir2dl4形成复合体的空间结构模拟，预测了它们之间相互作用的特征。 - The coding sequence of the two full - length cdnas were cloned by pcr , and inserted into expression vector pmet a between the downstream of a secreting signal peptide and the upstream of 6x histidine in the same reading frame with the coding sequence . the secreting recombinant expression vectors pmet a b / abp2304 and pmet a a / abp780 were constructed and transformed into pmad16 with lici transformation
利用pcr技术将上述两个全长cdna的编码区克隆到表达载体pmet上，使之位于因子信号肽序列的下游， 6个组氨酸残基序列的上游，且与之同框，分别构建成融合蛋白分泌表达载体pmet b abp2304和pmet a abp780 。 - We obtained the full length gene of hbfgf coding sequence with pcr , and adjusted the g + c content according the software dnasisv2 . 5 , and replaced the cys78 and cys96 with serines by site - directed mutagenesis . 2 . sequence result suggested one of the recombinant is correctly synthezied and cloned
用pcr方法合成了hbfgf编码区全长，其中前20个氨基酸的g + c含量按暨南大学硕士学位论文：大肠杆菌表达重组hbfgf结构和功能优化摘要照计算机软件计算的结果进行了调整，第78和96位上的半眯氨酸被突变为丝氨酸。 - 2 . firstly , the vector pcambia2301g with two gus reporter genes was constructed . then one gus was substituted with the coding sequence of dsg 10 obtained by pcr , and pcambia2301g - dsg10 was constructed . finally the pcambla2301g - dsg10 was introduced into agrobacterium tumefaciens using the freeze - thaw method 3
2 、首先构建了中间载pcambia2301g ，再用它和dsg10构建了可在植物中高效表达dsg10的载体pcambia2301g - dsg10 ，并将该质粒导入根癌农杆菌( agrobacteriumtumefaciens ) eha105中。 - Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3 . the recombinant plasmid was transformed to e . coli bl21 and the gst / mpem fusion protein was induced to express with iptg . the fusion protein was purified by affinity chromatography
方法： pcr扩增小鼠pemcdna编码序列，将它克隆到含有gst的原核表达质粒载体pgex - 4t - 3上，转化大肠杆菌bl21 ，诱导表达gst mpem融合蛋白，通过亲和层析，获得初步纯化的产物，以羟胺切割验证其一级结构。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。 - Rlean meat percentage is one of the most important economic traints in pig breeding programs . myostatin is a negative regulator of skeletal muscle growth . null or low activity of myostatin , individual muscle of mutant amimals would show a large and widespread increase in skeletal mass . myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle . the phenotype was termed double muscling . in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait , we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it
肌生成抑制素( myostatin ，即mstn )是近几年来( mcpherrona . c等， 1997 )发现的骨骼肌生长的负调控因子，它主要在骨骼肌中表达。其活性的丧失，会引起动物肌肉的过度发育，肌纤维直径变大或肌纤维数增加，表现为双肌症状。肌生成抑制素研究的突破将对猪、肉鸡、肉牛等畜禽生产性能的提高具有特别重要的意义。 - In addition , the nt - 3 and nt - 4 coding sequences were used to resolve the taxa problems of the giant panda and the red pandacaizurus fulgens ) , combining with mtdna genetic marker and other traditional taxonomic data . the result suggested that the giant panda should be classified as a separate subfamily within ursidae , while the red panda should be included in a monotypic family ailurdae
4基因的保守性，结合线粒体dna上的序列和其它传统分类学资料，对大熊猫及小熊猫的分类地位进行了探讨，认为应将小熊猫单独列为一科，大熊猫与小熊猫关系较远，应划入熊科。