we amplified aroa, aroc, arob of common pathway, csra and its flanking sequence of global regulation network from e . colt, and kan resistant gene from plasmid pet28a . 4 . centerpiece of this 在bioflo3000bateh/continuousbioreaetor(sl)进行发酵试验结果证实,发酵液中副生杂酸较少,产品经初步纯化后纯度较高,可达93%。
several enzymes that indirectly affect pep are also regulated by csra . thus, a csra mutation not only causes a significant elevation in intracellular pep, but also breaks cellular intrinsic metabolic coordination and channel more carbon flux to phenylalanine biosynthesis 另外,一些间接影响pep的酶也被csra所调控,因此csra的敲除不但可以使细胞内的pep的量大大增加,而且打破了细胞固有的生理代谢协调,使碳代谢流尽可能多的流向苯丙氨酸的生物合成方向。
several enzymes that indirectly affect pep are also regulated by csra . thus, a csra mutation not only causes a significant elevation in intracellular pep, but also breaks cellular intrinsic metabolic coordination and channel more carbon flux to phenylalanine biosynthesis 另外,一些间接影响pep的酶也被csra所调控,因此csra的敲除不但可以使细胞内的pep的量大大增加,而且打破了细胞固有的生理代谢协调,使碳代谢流尽可能多的流向苯丙氨酸的生物合成方向。
the global regulator csra of e . coli is a specific mrna-binding protein . csra negatively regulates several metabolic pathways that are induced post-exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism; positively controls gene expression within the glycolytic pathway; and also csra modulates the levels of enzymes that participate directly in pep metabolism csra是整体调控网络的调控基因,可负调控指数生长后期诱导的一些代谢途径,包括糖原的生物合成、糖原的分解代谢、糖异生,而对糖酵解的一些重要基因的表达则执行正调控功能,csra也调控直接参与pep代谢的三个酶的活性水平。
the global regulator csra of e . coli is a specific mrna-binding protein . csra negatively regulates several metabolic pathways that are induced post-exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism; positively controls gene expression within the glycolytic pathway; and also csra modulates the levels of enzymes that participate directly in pep metabolism csra是整体调控网络的调控基因,可负调控指数生长后期诱导的一些代谢途径,包括糖原的生物合成、糖原的分解代谢、糖异生,而对糖酵解的一些重要基因的表达则执行正调控功能,csra也调控直接参与pep代谢的三个酶的活性水平。