case of myiasis caused by the larva hypoderma bovis 牛皮蝇幼虫所致蝇蛆病1例1
and hypoderma de not . synthetical taxology )部分种进行较系统的研究。
the expression products were tested by wester-blotting, it demonstrated the fusion proteins can be recognized by positive serum of hypoderma sp 通过westem?blotting分析表达产物,表明表达产物具有免疫活性,能被制备的阳性血清所识别。
total rna was extracted from the second stage larve of hypoderma sp, the 713bp of hypodermin c ( hc ) and 700bp of hypodermin a ( ha ) gene were amplified by rt-pcr 2 从皮蝇二期幼虫中提取获得总rna,利用rt-pcr技术扩增获得了大小为713bp的hypoderminc和大小为700bp的hypodermina基因。
6 . hc and ha antigens were separated by sds-page from nature hypoderma sp larve, then immunized rabbitsrespectivelly, hc and ha positive serum were preparated . 7 从天然皮蝇幼虫中提取了皮蝇抗原,通过sds一page分离获得了hypoderminc和hypodermina天然抗原,用此抗原免疫家兔,制备了皮蝇hypoderminc和hypodermina阳性血清。