Construction and packing of replication - defective adenovirus vectors expressing hcv structural proteins will play an important role in research of hcv and vaccination . hcv infection often result in persistent infection 构建并包装表达hcv结构基因的复制缺陷型腺病毒载体,在丙型肝炎的防治和hcv的研究中具有重要意义。
3 . using clamp technique , ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig 供肝冷保存时,采用血管夹技术lamptechnique )经门静脉灌注携带融合基因hllctla4dg的重组腺病毒,于术后3天、 7天能定性检测到hllctla4ig在受体外划血卜1 。