- [网络] 转变
- transformation action: 变压作用，变换作用
- transformants: (transformant 的复数) n.[生]转化株(指已经转化的细菌细胞)
- transformation analysis: 转换分析
- transformance: [网络] 转换式表演
- transformation and search question analysis: 变换与搜寻问题分析
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transformate中文什么意思
- [网络] 转变
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例句与用法
- degenerate oligonucleotides to highly conserved regions of cucumis melo 1-aminocyclopropane-1-carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh, which was cloned into plasmid vector pmd-18-t . the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied, using pollen tube pathway transformate cucumis melo l . cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本实验以河套蜜瓜果肉基因组dna为模板，用甜瓜acc氧化酶基因特异寡核苷酸链为引物进行pcr扩增，得到128bp的扩增产物。将得到的扩增产物克隆到质粒载体pmd-18-t上，筛选反向克隆，然后将其反向构建到植物表达载体pbinyxw的camv35s启动子和tmv增强子“”的下游，构建成反义表达载体pbinya。并在对河套蜜瓜授粉受精生物学研究的基础上，通过花粉管通道法转化河套蜜瓜，共获76颗瓜，并进行了硬度和含糖量的分析。 - the engineering bacterium which carried bcih i-chi and i-glu cdna was pcg-ii . two methods of agrobacterium-mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time; compared agrabactenum-mediated method with gene gun method, the transformation frequency of the former was 16.7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用携带有几丁质酶基因和-1、3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16.7，基因枪法的转化率为50，因此可能基因枪转化法更适于龙牙百合的遗传转化。