Identification of znf219 nuclear localization signals 219基因核定位信号序列的确定
Both map2 and sap2 have two typical ap2 domains and a putative nuclear location signal , kksr Map2和sap2内部都含有两个典型的ap2结构域,并且n端都具有高度保守的核定位信号。
Similar to ap2 in arabidopsis , hap2 have a highly basic 10 - amino acid domain that includes a putative nuclear located sequence kksr 类似于拟南芥的a类基因apz , hapz亦含有10个氨基酸长的高度碱性的区域,该区域具有核定位信号kksr 。
At the same time , the c - terminal expressing plasmids of hcap - e and hcap - c that attached the kozac sequence and the nuclear localization signal were also constructed 同时也构建了附加kozac序列和核定位信号的hcap - e和hcap - c的c -末端真核表达质粒。
To identify the transfected cells , we also constructed the gfp expressing plasmids which also attached the kozac sequence and nuclear localization signal to the gfp gene and the plasmids served as a selective marker for the transfected cells 为了区分被转染的细胞,以附加kozac序列和核定位信号的gfp ( pcdna3 . 1 + kg )作为被转染的细胞的标记。