modulations,antigenic 《英文msh词典》Modulations,Antigenic ; [入口词] Modulations,Antigenic ; [主题词] Antigenic Modulation ; [英文释义] Loss of detectable antigen from the surface of a cell after incubation with antibodies. This is one method in which some tumors escape detection by the immune system. Antigenic modulation of target antigens also reduces the therapeutic effectiveness of treatment by monoclonal antibodies.
Western-blotting result demostrated rhpf4 had specific reaction with rabbit anti-hpf4 antibody . our system improve the expression level of r hpf4 by 80 fold compared with pt7-7-r hpf4 . after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4 . our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells 我们构建的rhpn原核高效表达系统经mpage及凝胶密度扫描分析结果表明,rhpf4表达量占菌体总蛋白量的2530,较原表达克隆pt77rhpf4提高了近80倍,经快速高效的包涵体分高纯化工艺和复性工艺,rhpf4具有野生蛋白活性。