hyperosmotic造句
例句与造句
- This structure increased obviously following hyperosmotic stimulation
高渗刺激后， edas的数量明显增多。 - While it is not clear that whether son astrocytes respond to hyperosmotic stimuli and what ' s the relationship between them and neurons
但是，关于胶质细胞在高渗刺激状况下，是否参与渗透压的调节及其与神经元的关系的研究尚未见报道。 - Treat primary cultured neurons and astrocytes with hyperosmotic stimulation . immunofluorescence was used to study the expression of cx43 and cx32 in son neurons and astrocytes
应用westernblot技术检测高渗刺激后视上核缝隙连接蛋白（ connexin ， cx ） 32和43含量。 - After hyperosmotic treatment , cx43 - li granules increased quickly on the plasm of the cultured astrocytes and cx32 - li increased apparently in the cultured neurons
而对培养的神经元和星形胶质细胞施予高渗刺激，星形胶质细胞膜上cx43样阳性颗粒迅速增多，神经元cx32表达明显增强。 - Objective : in this study we try to simultaneously investigate the response of neurons and astrocytes of rats following hyperosmotic stimulation and focus attention on the role of neuronal - astrocytic complex in the osmotic reglution
目的：本研究试图从整体水平共同观察神经元和ast对高渗透压刺激的反应，探讨神经胶质细胞?神经元复合体在渗透压调控过程中的作用。 - It's difficult to find hyperosmotic in a sentence. 用hyperosmotic造句挺难的
- 5 , after being treated with hyperosmotic stimulation , the cultured astrocytes and microglias showed a fast increase of intracellular calcium concentration followed by a decrease . the [ ca2 + ] j of astrocytes decreased slower than that of microglias . according to the r
根据实验结果，得出以下结论： l 、高渗刺激可以引起大鼠son内的星形胶质细胞和小胶质细胞快速的反应，星形胶质细胞的反应要早于神经元。 - Based on the previous researches , the present study investigated the response of son neurons and glias ( astrocytes and microglias ) to hyperosmotic stimulation and their relationship to find out the roles of glias in regulating hyperosmotic stimulation
因此在以往研究的基础上，本课题通过观察视上核神经元和胶质细胞(星形胶质细胞和小胶质细胞)对高渗刺激的反应及其相互关系，探讨视上核胶质细胞在高渗刺激后调节过程中的作用。 - Conclusions : the present results indicated that the brain areas involving in the heperosmotic regulation mainly located in mvz son and pvn . the neurons and astrocytes might be very active following hyperosmotic pressure and n - asc as a functional unit might serve to modulate osmotic pressure . there was reciprocal osmoregulation pathways between the mvz and son or pvn in the brain
结论：参与大鼠高渗性渗透压调节的中枢主要位于mvz 、 son和pvn等核团，神经元和ast以n asc的形式共同参与渗透压调节反应，体内存在mvz和son或pvn之间往返的渗透压调节通路。 - Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium . osmoregulation positions in the brain , reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ) , oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos , glial fibrillary acidic protein ( gfap ) , tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining , immuno - electronic microscope , wga - hrp retrogradely tracing and cell culture methods . results : ( 1 ) fos positive neurons within the mvz , parabrachial nuclei , locus ceruleus , pvn , son , subfomical organ increased markedly
方法：通过给予大鼠饮用3氯化钠或提高培养基渗透压浓度的方法复制高渗刺激模型，主要采用抗fos 、胶质原纤维酸性蛋白( gfap )和酪氨酸羟化酶( th ) (或加压素? vp )免疫组织化学多重染色、免疫电镜、 wga - hrp束路追踪结合免疫组织化学多重染色、细胞培养等实验方法，系统观察了中枢参与渗透压反射的调控部位、下丘脑视上核( son )神经元? ast超微结构的变化、延髓内脏带( mvz )和son及下丘脑室旁核( pvn )之间往返投射通路和神经元的性质及其与ast的关系、培养神经元和ast内钙波的变化。 - An acellular dermal matrix was prepared from allogenic skin by removing epidermis with a hyperosmotic salt solution and cross - linking with glutaralaldehyde , then clear away acellular components in dermis with naoh - maceration . the light and sme observation of the acellular dermal matrix revealed that the epidermis and cellular component in dermis were eliminated
本研究共分四部分：第一部分无细胞真皮基质的制备用高渗盐除去异体皮肤的表皮细胞，经戊二醛交联后以低浓度naoh消蚀以除去真皮中的所有细胞成分，得到无细胞真皮基质。 - Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2 , signal molecules , skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ) . radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation . ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method
应用免疫组织化学方法光镜下观察高渗刺激后，大鼠视上核胶质细胞(星形胶质细胞和小胶质细胞)受体( nmdar2 ) 、信号分子、骨架蛋白及缝隙连接蛋白的表达的时空变化；应用放免测定检测高渗刺激前后血浆中vp含量。 - We injected carbennoxolone , a gap junction blocker , into the lateral ventricle , which was followed by hyperosmotic stimulation , the immunohistochemical staining of neurons and astrocytes and vp content in plasm were studied . western blot was performed to detect the content of cx43 and cx32 in son following hyperosmotic stimulation
侧脑室注射缝隙连接阻断剂甘珀酸( carbenoxolone ， cbx )后，再给予高第四军医大学博士学位论文渗刺激，观察视上核胶质细胞和神经元免疫组化反应的改变，并检测血浆vp含量的变化。 - 3 , vp content in plasma increased significantly 45min after hyperosmotic stimulation . when pre - injected carbennoxolone , a gap junction blocker , into the lateral ventricle , followed by hyperosmotic stimulation , vp content remained the base line . the expression of gfap - li astrocytes showed no difference , while that of fos - li neurons decreased significantly
门）高渗刺激后扔dn ，血浆中p含量明显升高，若事先经侧脑室注入cbx后再给高渗刺激，血浆中vp含量不升高，视上核内星形胶质细胞gfap阳性反应与单纯高渗组无差别，而fos阳性神经元明显减少。 - Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz , pvn and son respectively ; ( 2 ) under electronic microscope , the astrocytic processes connected closely with the dendrites or axons of the neurons , where the bilateral membranes became thick . we call transiently it electron - dense areas ( edas ) . the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely , wga - hrp was microinjected into the unilateral son , pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method , the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz , son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution , intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
脑内gfap阳性结构也明显增多，其分布与fos阳性细胞分布基本一致，表现为胞体肥大、突起粗长； ast紧密包绕在神经元周围形成神经元- ast复合体( n - asc ) ；在mvz 、 pvn和son三重免疫组化染色切片上可见到fos + th + gfap +第四军医大学博士学位论文和fos vp gfap三重标记asc ； （ 2 ）免疫电镜下son内星型胶质细胞突起与神经元树突或轴突之间接触部位出现增厚的膜结构一电于致密区（ edas ） ，高渗刺激后数量明显增多： （ 3 ）将们个mp注入大鼠一侧n卜、卜卜或孤束核（ ws ） ，分别在延髓内脏带（ mvz ） 、 so和pvn内出现fos hrp th 、 fos hrp八p三重标记神经元和gfap阳性标记ast形成的n asc ； （ 4 ）高渗刺激使培养神经元和ast内钙水平先升高后降低，最后维持在比高渗刺激前稍高的静息钙水平上。