Always use automatic pipettes or pipetting devices . no mouth pipetting is permitted 必须使用自动吸移管或吸移工具,不可使用口用吸移管。
To make pipetting easier , we will use an enzyme master mix that already contains the appropriate buffers and the two restriction enzymes 为了能在吸取液体时操作方便,我们会利用已经含有适当缓冲溶液与两种限制酶的酵素反应混合液来操作。