A new method for intrinsic terminator prediction based on rnall , an rna local secondary structure prediction algorithm developed recently , and two u - tail score schemas are developed . by optimizing three parameters thermodynamic energy of rna hairpin structure , u - tail t weight , and u - tail hybridization energy , the method can recognize 92 . 25 of known terminators while rejecting 98 . 48 of predicted rna local secondary structures in coding regions negative control as false intrinsic terminators in e . coli . this method was applied to scan the genome of synechococcus sp 在过去二十年中,不少研究者已开始研究如何用计算方法来预测转录终止信号,如brendel和trifonov的双核苷酸分布矩阵法dinucleotide distribution matrix carafa等的统计方法transterm和rnamotif法等,这些方法都从不同方面考虑了rna二级结构和u -尾部的特征,而gester的预测模型则设定rna二级发夹结构是不依赖终止子的唯一因素。
At present , in various way of structure prediction of new sequence , there lie such problems : in the process of modeling a new sequence , the alignment between target sequence and model sequence we get is not very accurate , so it has a negative effect on the protein structure predicted ultimately 目前对于预测一个新序列的蛋白质结构的各种方法中,大多存在这样的问题:在对一个新序列构建模型的过程中,生成的查询序列和目标序列的排列不够准确,进而影响了最终预测出来的蛋白质结构的准确性。
The gene was 1668bp in length , encoding the f protein composed of 553 amino acids . sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids , six potential glycosylation sites and thirteen cysteines . a sequence region of basic amino acids , rrqrrf , was found at the f , - f2 cleavage site , indicating that f4ge9 was a typical virulent strain 序列分析和二级结构预测表明, f _ ( 48 ) e _ ( 9 )株f蛋白含有3个分别由25个氨基酸组成的疏水区,存在6个潜在的糖基化位点, 13个半胱氨酸残基,裂解位点区域的氨基酸序列为rrqrrf ,说明f _ ( 48 ) e _ 9株是一株典型的强毒株。
In this paper , we first analyze the validity of a quasiphysical algorithm for protein structure prediction , and show that there exists much randomness in obtaining a valid folding structure using this algorithm , then give anjudgment criterion for the bond " interfere " in folding structure , and propose modifying schemes used to improve the validity of the quasiphysical algorithm 摘要对预测蛋白质空间结构的拟物算法的有效性进行理论分析,证明用该拟物算法求得合法的结构存在较大的随机性;给出折叠结构发生冲突的判断条件和提高拟物算法有效性的一些修正方案。