Fos paper consists of three parts : first , we analyze the main problems of existing intelligent item bank systems and the act ' ual requlrements and then introduce agent and develop and design the system tising agent - oriented analysis 主要做了以下的工作:第一,介绍智能题库系统的概念和结构,分析已有智能题库系统的主要问题,引入agent技术,提出开发基于agent技术的智能题库系统( bais )的设计思想。
It consists of fusiform neurons with intensely expressed neuropeptides in the fibers , terminals , and neuronal somata . functional connections between the mrd and hippocampus , amygdala and the basal nucleus of meynert were identified by c - fos expression method [ 14 ] Nmdar在谷氨酸中枢神经系统的许多功能上具有关键作用, nmda受体激活后可引起神经元细胞膜对na + 、 k +和ca2 +通透性增强,产生兴奋性突触后电位。
C - fos probably regulates the steriodogenesis of interstitial cells directly and effects the communication of information between follicular cells and germocytes . e2 probably induces the activity of c - fos in testis . the concentration of t is correlated with the express of c - fos C厂os产物还可以直接作用于问质细胞调控其产生类固醇激素,并参与支持细胞与生精细胞的信息传递的含量与c fos的表达也有直接关系。
Thus , the present study is to investigate if these mglurs are f unctionally involved in the process of activation of nociceptors as iglurs do by using formalin tests combined with a behavioral and fos expression examinations . there were two parts of experiments 本实验分为两部分,第一部分是在实验大鼠同时观察两个痛反应指标,即福尔马林脚掌注射引起的行为学改变以及福尔马林脚掌注射引起的脊髓背角fos蛋白的表达。
Results show that not only most types of rim - fos can be simulated by the simulation and testing system , but also any kind of which can be tested automatically . also the system can work as soon as power on , while free of the disturbance of environment and power fluctuation of the light source 结果表明,该系统不但可完成rim - fos特性仿真和特性测试功能,而且还可以开机即测且不受环境光干扰和光源功率波动的影响,测试稳定性好,重复性较高。
During this annual conference to be held between june 23 and 25 , the heads of customs administrations from 166 wco members worldwide will consider , among other issues , the adoption of the " framework of standards to secure and facilitate global trade " ( fos ) developed by the wco s high level strategic group 于六月二十三日至二十五日年会举行期间,来自世界各地的166个世界海关组织成员的海关首脑将讨论多个事项,其中包括采纳一套由世界海关组织高层策略组发展的保障及利便国际贸易标准。
In the first part , both behavioral responses and fos expression in dorsal horn induced by peripherally injected formalin were observed . animals were divided into three groups ( n = 5 ) : one of them was control with injection of vehicle ( hepes ) into the plantar aspect of the left hindpaw ; and two of them were experimental groups with injection of selective mglur , and mglur5 antagonists , cpccoet or mpep , respectively 实验组动物山西医科大学的一侧后脚掌分别注入20仍级硕士学位论文mg1ur 、 、 mglur 、的拮抗剂cpccoet ( 1oon二01 ) / m尸ep ( 50nmol ) ,对照组则在一侧脚掌注入缓冲液hpeps (两种拮抗剂均用hpeps溶解) 。
The present study , ( d by using immunohistochemical single , double or triple staining method , showed the expression , relationship and distribution pattern of fos - protein , gfap or th in rat cns , investigated the plastic response and relationship of rat lumbar spinal cord as and neurons to pain induced by the unilateral tibia and fibula fracture ; ( 2 ) by using a double immuno - electron - microscopic method , investigated the ultrastructural characters of junction areas between neurons and as in the dorsal horn of rat lumbar spinal cord following the unilateral tibia and fibula fracture ; ? after intrathecal application of the carbenoxolone , a gap junction blocker , recorded the paw withdrawal thermal latency and compared with control 在脑干gfap - li星形胶质细胞主要分布于mvz内的孤束核( nts ) 、腹外侧延髓( vlm )以第四军医大学博士学位论文及两者之间的中间带( irt )上。三叉神经脊束核尾侧亚核( vc ) 、外侧楔柬核( ecu ) 、蓝斑( lc ) 、臂旁外侧核( lp ) 、中缝大核( rmg ) 、中脑导水管周围灰质腹外侧区( vipag ) 、中缝背核( dr )等部位也出现一定数量的gfap阳性细胞。 f 。
Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium . osmoregulation positions in the brain , reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ) , oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos , glial fibrillary acidic protein ( gfap ) , tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining , immuno - electronic microscope , wga - hrp retrogradely tracing and cell culture methods . results : ( 1 ) fos positive neurons within the mvz , parabrachial nuclei , locus ceruleus , pvn , son , subfomical organ increased markedly 方法:通过给予大鼠饮用3氯化钠或提高培养基渗透压浓度的方法复制高渗刺激模型,主要采用抗fos 、胶质原纤维酸性蛋白( gfap )和酪氨酸羟化酶( th ) (或加压素? vp )免疫组织化学多重染色、免疫电镜、 wga - hrp束路追踪结合免疫组织化学多重染色、细胞培养等实验方法,系统观察了中枢参与渗透压反射的调控部位、下丘脑视上核( son )神经元? ast超微结构的变化、延髓内脏带( mvz )和son及下丘脑室旁核( pvn )之间往返投射通路和神经元的性质及其与ast的关系、培养神经元和ast内钙波的变化。
First , to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp , and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second , based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins , the concentration of the hab toxins could be detected 目的:构建一个含c - fos启动子和egfp报告基因的pegfp - c - fos重组质粒载体。体外转染膀胱癌biu - 87细胞后,利用赤潮毒素作用后细胞表达绿色荧光蛋白的变化来检测赤潮毒素,初步建立一种以细胞为基础受体水平的赤潮毒素检测方法。