插入 insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromission; dig; implant; inlay; interpose; intervene; interposition [数学] interpolation 把钥匙插入锁中 insert a key in(to) a lock; 用剑插入某人的胸膛 thrust one's sword through sb.'s bosom; 有时插入一个字可以改变全句的意义。 sometimes the insertion of one word can change the meaning of a whole sentence
Transformation efficiency is greatest with circular plasmids containing only small inserts of passenger dna 使用带有一小段客座dna的插入序列的环状质粒进行转化,较率会最高。
The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod 结果表明同源性分别达到98和97 ,并且在ha1切割位点有多个碱性氨基酸的插入序列,证明其为强毒株。
Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis . we took a pcr method to clone the cdna of osdd2 gene 使用克隆的边端插入序列为探针对水稻(中花11品种)进行southern杂交分析,表明osdd2基因在水稻基因组中是以单拷贝存在。
Methods : the two pairs of designed primers were based on pzp3 a and hcg p - ctp109 - 145 cdna sequences . the pzp3 a - hcg p - ctp109 - 145 chimera was amplifiled by overlapping pcr . the chimera was cloned into ppic9k plasmid and transformed into e . coli dh5 a 结果: 3步pcr扩增出pzp3 - hcg - ctpdna片段,插入到载体质粒ppic9k的克隆位点,获得重组ppic9k - pzp3 - hcg - ctp表达质粒,测序结果显示插入序列与设计预期完全一致。